Adult stem cells are the resident stem cells of tissues responsible for maintaining tissue homeostasis. These cells reside in quiescence for prolonged periods. Quiescence is a reversible G0 state, important in preserving stem cell potency and their functions during tissue homeostasis and tissue repair in the event of injury. Cellular quiescence is characterized by the downregulation of cell cycle-related genes and reduced RNA and protein content. Furthermore, quiescent stem cells exhibit lower metabolic activity, relying on glycolysis to meet their energy demands. Quiescence was long considered an inactive state, but recent studies have demonstrated that adult stem cells are highly responsive to extrinsic stimuli. Indeed, adult stem cells are under active regulation during the quiescence-to-activation transition and maintenance of the quiescence state. Such mechanisms include the expression of quiescent-specific microRNAs, transcriptional regulation of cell cycle-related genes, and chromatin modifications. The Cheung Laboratory is highly interested in understanding the post-transcriptional mechanisms underlying this process using muscle stem cells (also known as satellite cells), the adult stem cells of skeletal muscle as a model of quiescence.